D3922 thermo fisher scientific

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WebNov 8, 2024 · The cells were co-stained with BODIPY™ 493/503 for lipid droplets (D3922; Thermo Fisher Scientific), LysoTracker™ Deep Red (L12492; Thermo Fisher Scientific), or MitoTracker™ (M7510; Thermo Fisher Scientific). Nuclei were blue-stained with 4′,6-diamidino-2-phenylindole (DAPI). Tumor xenograft. All animal study procedures were … WebDec 12, 2024 · Lipophilic green-fluorescent BODIPY 493/503 (D3922, Thermo Fisher Scientific, Waltham, MA, USA) was used for lipid staining. SkMs were seeded at a density of 1000 cells/cm 2 and treated as indicated.

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WebOct 21, 2024 · LipidSearch™ (Thermo Fisher Scientific) was used to make lipid peak assignments in the single cells. Only peaks above a 1E4 intensity threshold and within 5 ppm of the calculated m / z value were selected. ... (#D3922, Invitrogen, USA) in PBS and incubated in the dark for 15 minutes at 37 °C with 5% CO 2. After incubation, cells were … WebBioactive Small Molecules D3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) … fish eye lens 70mm

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WebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … WebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. fisheye lens bathroom pics

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Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell …

WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … WebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … fisheye lens app iphoneWebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … can a person lose their vrs retirement

"WebFeature papers are submitted upon individual invitation or recommendation by the scientific editors and must receive positive feedback from the reviewers. ... Alternatively, for BODIPY staining, 3D spheroids in 6 super-low-attachment well dishes were incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h ... " - D3922 thermo fisher scientific

D3922 thermo fisher scientific

Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell ...

Web货号: D3922 相关应用: Cellular Imaging 技术支持客户服务收藏夹产品概述推荐产品文件常见问题解答引用和文献 BODIPY™ 493/503 具有非极性结构、吸收波长较长且发荧 … WebAug 1, 2024 · For BODIPY lipid staining [6–8], 4% paraformaldehyde fixed 3D sphenoids were incubated in 0.1% BODIPY (# D3922, Thermo Fisher Scientific), 0.1% DAPI (#D523, Doujin, Japan) and 0.1% phalloidin (#20,553, Funakoshi, Japan) in phosphate buffered saline containing 3% bovine serum albumin for 3 hrs.

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WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature. WebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) …

WebMeasurement Range: -50 to +392°F/-45 to +200°C NSF® Certified One-button operation 3 mm tip 5"/12.7 cm stem Shatterproof Food-safe ABS plastic with BioCote® Stainless … Web493/503 (D3922; Thermo Fisher Scientific) for 30min, followed by thrice PBS washes. The IEC were observed with a laser scan- ... (Thermo Fisher Scientific) and blocked with 8% (w/v) skimmed milk in Tris buffered saline þ Tween 20 (TBST) buffer (20m M Tris–HCl, pH7·5,150mM sodiumchloride,0·1%Tween20)atroomtemper-

WebJun 18, 2024 · Cells were cultured in 12-well plates and treated with the corresponding treatments for the required period, washed twice with PBS, and incubated with 5 µg/ml Bodipy 493/503 (D3922; Thermo Fisher Scientific Waltham, MA, USA) for 30 min, followed by 3 PBS washes, hepatocytes were observed with a laser scanning confocal … WebSU24 - Scientific research and development Uses advised againstNot for consumer use. Details of the supplier of the safety data sheet Manufacturer/Supplier 24 hour Emergency …

WebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS.

WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … fisheye lens 360 wireless cameraWebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... fisheye lens by itselfWebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... can a person live without a spineWebJul 23, 2024 · Thermo Fisher: Cat #D3922: Donkey anti-mouse IgG (H + L) secondary antibody alexa Fluor® 488 conjugate: Thermo Fisher: ... (D3922, Thermo Fisher Scientific, California, USA) staining solution in the dark for 15 min at 37°C. After washing 3 times with PBS, cells were stained with theirs surface markers for 30min at 4°C. Data … can a person on a ventilator hearWebInvitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) Catalog number: D3922 Related applications: Cellular Imaging Technical … can a person on bail travel abroadWebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … can a person live with 1 kidneyWebIn order to identify podocyte proteins that could perform as a similar or better biomarker for the presence of CPF (s), or are involved in the mechanism of action of CPF (s), we here followed a proteomics approach by analyzing proteomes of hPod exposed to presumably CPF-containing plasmas from patients with FSGS. fisheye lens canon m3